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Image Search Results
Journal: PLoS Pathogens
Article Title: Vibrio parahaemolyticus Effector Proteins Suppress Inflammasome Activation by Interfering with Host Autophagy Signaling
doi: 10.1371/journal.ppat.1003142
Figure Lengend Snippet: A and B. Activated endogenous Cdc42 was analyzed by GST-PAK PBD pull-down and immunoblotting with anti-Cdc42 antibody. A. The cell lysates of NLRP3-deficient BMMs infected with Δ tdhAS or Δ tdhAS Δ vopS mutants for one hour were loaded with GDP or GTP-γS. B. Cell lysates of NLRP3-deficient BMMs infected with Δ tdhAS , Δ tdhAS Δ vopS mutants complemented by WT vopS or vopS H348A for 1 h were loaded with or without GTP-γS. C. Effect of VopS H348A mutant on caspase-1 activation and IL-1β processing in infected NLRP3-deficient BMMs with Δ tdhAS Δ vopS mutant or complemented strains by wild-type vopS or vopS H348A. D. Effect of VopS H348A mutant on release of IL-1β from infected NLRP3-deficient BMMs with Δ tdhAS Δ vopS mutant or complemented strains as in C . Data are presented as the means ± SD of triplicate samples. *p<0.05. E. Effect of VopS H348A mutant on release of IL-1β from infected NLRP3-deficient BMMs with Δ tdhAS Δ vopQS mutant or complemented strains as in C . Data are presented as the means ± SD of triplicate samples. *p<0.05.
Article Snippet: The following antibodies were obtained commercially: rabbit anti-mouse caspase-1 (sc-514, Santa Cruz), goat anti-mouse IL-1β (AF-401-NA, R & D Systems), rabbit anti-mouse IL-18 (5180R-100, BioVision), rabbit anti-mouse LC3 (PM036 and M152-3, MBL), rabbit anti-mouse ATG5 (#8540,
Techniques: Western Blot, Infection, Mutagenesis, Activation Assay